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KMID : 0545120120220111467
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 11 p.1467 ~ p.1470
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Comparison of Multilocus Sequence Typing (MLST) and Repetitive Sequence-Based PCR (rep-PCR) Fingerprinting for Differentiation of Campylobacter jejuni Isolated from Broiler in Chiang Mai, Thailand
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Prapas Patchanee
Chomporn Chokboonmongkol
Karl-Hans Zessin
Thomas Alter
Sarinya Pornaem
Nipa Chokesajjawatee
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Abstract
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We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
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KEYWORD
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MLST, rep-PCR, Campylobacter, broiler
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